Growth of Mycobacterium lepraemurium in Cell-Impermeable Diffusion Chambers
نویسندگان
چکیده
منابع مشابه
Growth of Mycobacterium lepraemurium in Cell-Impermeable Diffusion Chambers.
Successful growth of Mycobacterium lepraemurium has been achieved by use of a specialized diffusion chamber technique. The cell-impermeable porous chambers were maintained in animals for periods up to 50 days with and without macrophages and LM cells. A generation time of 6 to 8 days was found for the acid-fast bacilli in chambers containing macrophages when maintained in the mouse. Also, cell-...
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Mycobacterium lepraemurium cells were found to multiply in normal mouse peritoneal-derived and bone marrow-derived macrophages in vitro. Whereas activated peritoneal-derived macrophages demonstrated marked bacteriostasis for M. lepraemurium, significant bactericidal activity was exhibited by activated bone marrow-derived macrophages. However, only a small proportion of the bacteria were killed ...
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A cell-impermeable diffusion chamber technique has been developed that lends itself to growth studies of Mycobacterium lepraemurium. This technique, in which the organism grows within macrophage cultures inside the chambers that are maintained on monolayer cultures of macrophages, provides a method for a strict in vitro evaluation of antileprosy drugs without the influence of a multiplicity of ...
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The structural properties of the cell envelopes of Mycobacterium leprae and Mycobacterium lepraemurium were investigated by freeze-fracture, freeze-etching, and negative-staining techniques. Freeze-fracture split the cell wall and exposed the internal features of the peptidoglycolipid mycosidic filamentous network. The cell membrane was also split into two asymmetric faces. The external fractur...
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Successful growth of Mycobacterium lepraemurium was observed in cultures of mouse peritoneal macrophages. The optimal host cell maintenance medium was composed of 40% horse serum, 50% of the chemically defined medium NCTC 109, and 10% of a 1:5 dilution of beef embryo extract, supplemented with both liver extract and ferric nitrate. Multiplication of the bacilli was observed in 1 week and maxima...
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ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1971
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.3.1.127-132.1971